![]() However, AST of the individual bacterial isolates is crucial for species with acquired resistance determinants, such as extended spectrum beta-lactamase producing Enterobacteriaceae, Streptococcus pneumoniae, Pseudomonas sp., Staphylococcus sp. Empirical antibiotic therapy continues to be effective for some bacteria: for example, there has not yet been any reported resistance of Streptococcus pyogenes to penicillin. The performance of antimicrobial susceptibility testing (AST) of bacterial pathogens is an important task to determine susceptibility to the antimicrobial agents and to detect possible drug resistance in clinical microbiology laboratories. ![]() This review is focused on the methods and instruments in use both currently and in the foreseeable future, applicable to determine antimicrobial efficacy in clinical microbiology laboratories. Recently developed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introduced in recent years into laboratory practice, and methods based on microfluidics and microdroplets might be introduced in the near future. Hence, there is a drive to develop and introduce novel, faster, standardized, sensitive and specific methods with reliable results into routine microbiological laboratory practice. Nevertheless, the results cannot be obtained earlier than 48 h after receiving a sample, which may lead to prolonged use or overuse of broad-spectrum antibiotics. ![]() Conventional detection methods of bacterial resistance, such as disc diffusion, broth microdilution and automated instruments, are constantly widely used and primarily standardized. ![]() Rapid identification of pathogen and its resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment are essential for correct patient outcomes. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |